Gwang-Bum Im (Prof. Suk Ho Bhang’ group) in School of Chemical Engineering, Sungkyunkwan University reports a new platfo
- 공과대학
- Hit4146
- 2021-07-06
Gwang-Bum Im (Prof. Suk Ho Bhang’ group) in School of Chemical Engineering, Sungkyunkwan University reports anew platform which used both 2D and 3D keratinocytes’ performance to enhance wound closure.
Journal: Chemical Engineering Journal
Paper Title: 2D and 3D co-spatial compartmentalized patch to enhance the therapeutic efficacy of keratinocytes for wound closure
Contents: We report the fabrication of a 2D and 3D co-spatial compartmentalized patch (CSCP) for a microscale keratinocyte-engineering platform and a novel method for delivering keratinocytes to enhance wound closure compared to that achieved by the conventional keratinocyte delivery method. Micro-sized cylinders engraved within a polydimethylsiloxane (PDMS) substrate were fabricated to induce and trap 3D keratinocyte clusters. Simultaneously, the outer surface of the PDMS substrate was used as an area for 2D keratinocyte culture. As a result, the CSCPs could be simultaneously applied for both 2D and 3D in vitro keratinocyte culture and in vivo cell delivery. The 3D keratinocytes increased the cell density for delivery and angiogenic factor expression but decreased the cell proliferation, which was complemented by the high proliferation of 2D keratinocytes. Our in vivo results confirmed that the therapeutic efficacy of keratinocytes for wound closure was significantly enhanced when the cells were delivered in a 2D and 3D co-spatial manner.
DOI: https://doi.org/10.1016/j.cej.2020.128130
Gwang-Bum Im is a unified master's and doctor's course student under the supervision of Prof. Suk Ho Bhang in School of Chemical Engineering, Sungkyunkwan University (SKKU). His main research interests include the biomedical engineering, tissue engineering, and their applications. The recent work is based on the co-delivery of 2D and 3D keratinocytes through the PDMS patch and its therapeutic effect.
Scheme figure of 2D and 3D co-spatial compartmentalized patch (CSCP), co-delivery of 2D and 3D keratinocytes enhanced the wound healing effect