Research team led by Professor Uhm Sung-ho of Chemical Engineering improves detection efficiency of genetic bio-markers
- 공과대학
- Hit5491
- 2019-12-16
A research team led by Professor Uhm Sung-ho of the Department of Chemical Engineering/Polymer Engineering,
Significant improvement in gene bio-marker detection
- Development of the 'iPSR' technology to replace the Polymerase Chain Reaction (PCR) technology for the probe intensive ionized strand replacement (iPSR)
- In vitro diagnosis within a few minutes, high-performance genes can be determined simultaneously
- Propose new indicators for future disease prevention and customized treatment
The team led by Professor Uhm Sung-ho of the Department of Chemical Engineering/Polymer Engineering said it has developed an approach and system that has revolutionized the detection efficiency of cancer nucleic acid biomarkers under isothermal conditions. The study, which considered the process of detecting bio-markers from a chemical reaction epidemiological perspective, not only has significantly improved the detection limits of nucleic acid bio-markers, but also suggests applicability to all systems involving interaction of probes and targets.
The research was carried out with the support of the Postgenome Multi-Ministry Genome Project in Health and Medical Technology R&D Project hosted by the Ministry of Health and Welfare and the Korean Research Foundation under the Ministry of Education, Science and Technology, and was published on November 15 in the Analytical Chemistry, the most prestigious journal of analytical chemistry.
The team improved the detection efficiency of nucleic acid bio-marker of conventional isomerase chain reaction (PCR) based technology by up to 300 times by designing and synthesizing nucleic acid nanostructures that concentrate a number of probes based on nucleic acid nanotechnology. The polymerase chain reaction-based system, a conventional nucleic acid bio-marker detection technology, was only able to operate at the laboratory level due to the need for complex reagents and devices. The research team developed a Probe-localized Isothermal Strand Replacement (iPSR) approach that encompasses the advantages of isothermal strand replacement technology and also significantly improves detection limits. Not only does this provide a new direction for nucleic acid bio-marker et al. detection technology, but it is also possible to make universal use in many ways according to the approach of the chemical reaction dynamics perspective.
The target detection process for all bio-sensors consists of the target recognition of probes and the signalling of perceived information, and the typical nucleic acid bio-marker detection techniques currently developed focus on how PCR-based amplification of the target material itself or the amplification of the generated electro-chemical signal increases detection efficiency. In this study, we adopted an approach that fundamentally improves the effective frequency of collisions needed for the response between probes and target molecules that deviate from the existing framework and plunge at low concentrations. Assuming probe and target reactions as a chemical reaction, the fundamental view of reaction mechanics that improves the frequency of collisions between molecules not only significantly improved the detection efficiency of bio-markers but also suggests the possibility of universal application in all chemical reactions involving collisions between molecules. This technology will be applied to the development of new gene diagnostic chip technology through DNANO (CEO Lee Young-gu) and will soon be commercialized.
"We have presented a possibility of significantly improved gene detection without PCR in a relatively short period of time, and it is universally applicable to any system that uses nucleic acid detection," Professor Um said. "We will greatly contribute to patient-tailored treatment through rapid treatment prescription and real-time monitoring of drug efficacy in actual clinical diagnosis."